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TUMOR MICROENVIRONMENT LABORATORY

Seoul National University
College of Veterinary Medicine

RESEARCH INTERESTS

We have diverse interests in Cancer/Radiation Biology and Inflamatory Diseases.  Here are only some of the in-depth projects currently undergoing in the lab. Since we're trying to pursue 'TRANSLATION RESEARCH' (i.e., clinical implication & applicability), please take a look at 'Collaborators' section below for more information on our latest research interests.

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GO GET EM TIGER!
BLOCK MACROPHAGES!

Macrophages are well known for their protumoral roles. Recently we have reported that macrophages can exacerbate tumor hypoxia and aerobic glycolysis. If they can consume oxygen and glucose, how will they regulate T cell functions and immunotherapy? How can we block macrophages? What signaling in macrophages is responsible for this phenomenon? We have all the know-hows and the newest mouse lung cancer model (ALK overexpression) mimicking patients in the lab to address this question!

FLASH, THE NEW RADIOTHERAPY ON THE BLOCK!

In the clinic, we treat tumors with radiotherapy using LINAC (linear accelerator), proton radiotherapy, or carbon (particle) radiotherapy. In collaboration with Stanford University, US, we are exploring novel biological mechanisms (effects in the tumor microenvironment) from the latest radiotherapy technique called FLASH. This is ultra-fast dose rate ionizing radiotherapy, which can deliver 20 Gy (very high dose of ionizing radiation) in just a couple of seconds!

YOUR FAT , YOUR GENE IN MACROPHAGES!

We have recently created novel myeloid-specific knockout mice in hypoxia-inducible factor (HIF) signaling pathway and found that these mice exhibit anti-obese phenotype. We're currently investigating the molecular mechanisms and seeing that it is Hif-alpha gene in macrophages regulating the diet-induced obesity. We're expanding this idea to Cancer Biology to see how macrophage could regulate fat metabolism in cancer cells.

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BETTER MOUSE MODEL FOR BETTER  IMMUNOTHERAPY TO TREAT LUNG CANCER!

Current response rate for the latest immunotherapy (PD-1 or PD-L1 bloackdes) in lung cancer patients (NSCLC) is only about 30%. To increase the effectiveness of immunotherapy, we need to understand how immune cells function and interact with each other during the lung cancer progression. We have a novel strain of mouse lung cancer mimicking NSCLC patients (ALK overexpressed tumors) to address this.

SEEING IS BELIEVING!

We are interested in the real-time in vivo imaging of macrophages (and other immune cells such as T cells) infiltrating into tumors using the dorsal (and intracranial) window chamber techniques that we have established in our lab. We can powerfully manipulate cancer cells so that they can express reporters upon metabolic/stress conditions. In addition, we can study the mechanisms of various anticancer modalities (such as DNA repair inhibitors,  checkpoint blockades, and anti-angiogenic agents) in live mice.

ARE YOU BREATHING?
OXYGEN-SENSING IN MACROPHAGES!

We have recently reported that tumor-associated macrophages (TAM) can compete with cancer cells for oxygen and glucose. Does hypoxia-inducible factor (HIF) regulate this mechanism? Does this occur in other metabolic diseases such as obsity and cancer cachexia? We have novel strains of mice targeting HIF pathways in macrophages to address this!

PUBLICATIONS

Major publications only

Jan 23, 2024

DNA-dependent protein kinase regulates cytosolic double-stranded DNA secretion from irradiated macrophages to increase radiosensitivity of tumors

  • Oh T et al., Radiother Oncol (2024), 10.1016/j.radonc.2024.110111

Oct 27, 2023

FLASH Radiotherapy: A FLASHing Idea to Preserve Neurocognitive Function

  • Jo HJ et al., Brain Tumor Res Treat (2023), 11(4): 223–231

Nov 1, 2023

Sensing the oxygen and temperature in the adipose tissues - who's sensing what?

  • Kang GS et al., Exp Mol Med (2023), 10.1038/s12276-023-01113-x

Apr 30, 2023

DNA-DEPENDENT PROTEIN KINASE CATALYTIC SUBUNIT (DNA-PKCS): BEYOND THE DNA DOUBLE-STRAND BREAK REPAIR

Nov 10, 2022

EFFECTS OF ULTRA-HIGH DOSE RATE FLASH IRRADIATION ON THE TUMOR MICROENVIRONMENT IN LEWIS LUNG CARCINOMA: ROLE OF MYOSIN LIGHT CHAIN

Lee YR et al., Mol cells (2023), 46(4): 200–205

  • Kim YE et al., Int J Radiat Oncol Biol Phys (2020), S0360-0316(20)34500-4

  • Young-Eun Kim received 2012 Global PhD Fellowship, 2015 Keystone Scholarship (Dublin, Ireland), 2018 KSMCB Best Poster Award (Seoul, Korea)

  • Seung-Hee Gwak received 2019 Junior Investigator Award (Tumor Microenvironment Workshop, Florida, US)

  • Beom-Ju Hong received 2013 Global PhD Fellowship, 2015 Junior Investigator Award (ICRR, Kyoto, Japan), 2015 Excellent Poster Award (ICRR, Kyoto, Japan), 2017 Junior Investigator Award (Tumor Microenvironment Workshop, Florida, US)

Sep 28, 2020

Feb 15, 2019

TARGETED DEGRADATION OF TRANSCRIPTION COACTIVATOR SRC-1 THROUGH THE N-DEGRON PATHWAY​

TUMOR-ASSOCIATED MACROPHAGES ENAHNCE TUMOR HYPOXIA AND AEROBIC GLYCOLYSIS

  • Lee Y et al., Angew Chem Int Ed Engl (2020) 59(40), 17548-17555

  • Hoibin Jeong received 2016 POSCO CheongAm Graduate Fellowship, 2019 Best Research Paper in the Dept Life Science POSTECH

  • Jeong & Kim et al., Cancer Res (2019), 79(4):795-806

  • Hoibin Jeong received 2016 POSCO CheongAm Graduate Fellowship, 2019 Best Research Paper in the Dept Life Science POSTECH

  • Chan-Ju Lee received 2015 Young Investigator Award (ICRR, Kyoto, Japan)

  • Introduced at BioWorld, Editor's Choice

Jul 30, 2018

HIF-1A ACTIVATION IN MYELOID CELLS ACCELERATES DEXTRAN SODIUM SULFATE-INDUCED COLITIS PROGRESSION IN MICE

  • Kim et al., Dis Model Mech (2018) 11(7), pii:dmm033241

  • Young-Eun Kim received 2012 Global PhD Fellowship, 2015 Keystone Scholarship (Dublin, Ireland), 2018 KSMCB Best Poster Award (Seoul, Korea)

  • Jeongwoo Kim received 2016 Global PhD Fellowship

  • Sujin Yeo received 2014 Global PhD Fellowship

Dec 1, 2017

HYPOXIA-INDUCIBLE FACTOR-1A REGULATES MICROGLIAL FUNCTIONS AFFECTING NEURONAL SURVIVAL IN THE ACUTE PHASE OF ISCHEMIC STROKE IN MICE

  • Oncotarget (2017), 8(67), 111508-111521

  • Seoyeon Bok is currently doing her postdoctoral fellowship in Cornell University, US

Dec, 2016

TUMOR HYPOXIA AND REOXYGENATION: THE YIN AND YANG FOR RADIOTHERAPY

  • Hong et al., Radiat Oncol J (2016), 34(4), 239-249

  • Beom-Ju Hong received 2013 Global PhD Fellowship, 2015 Junior Investigator Award (ICRR, Kyoto, Japan), 2015 Excellent Poster Award (ICRR, Kyoto, Japan), 2017 Junior Investigator Award (Tumor Microenvironment Workshop, Florida, US)

Jul 1, 2016

RADIATION-INDUCED ESOPHAGITIS IN VIVO AND IN VITRO REVEALS THAT EPIDERMAL GROWTH FACTOR IS A POTENTIAL CANDIDATE FOR THERAPEUTIC INTERVENTION STRATEGY.

Kim & Jeon et al., Int J Radiat Oncol Biol Phys (2016), 95(3), 1032-1041

Jul 1, 2016

REAL-TIME TUMOR OXYGENATION CHANGES AFTER SINGLE HIGH-DISE RADIATION THERAPY IN ORTHOTOPIC AND SUBCUTANOUES LUNG CANCER IN MICE: CLINICAL IMPLICATION FOR STEREOTACTIC ABLATIVE RADIATION THERAPY SCHEDULE OPTIMIZATION.

  • Song & Hong et al., Int J Radiat Oncol Biol Phys (2016), 95(3), 1022-1031

  • Introduced as research highlight

  • Commented by Editor-in-Chief at PodCast

Aug 7, 2015

IN VIVO IMAGING OF ACTIVATED MICROGLIA IN A MOUSE MODEL OF FOCAL CEREBRAL ISCHEMIA BY TWO-PHOTON MICROSCOPY

Bok et al., Biomed Opt Express (2015), 6(9), 3303-3312

Feb 18, 2014

TRANSCRIPTIONAL ACTIVATION OF HYPOXIA-INDUCIBLE FACTOR-1 (HIF-1) IN MYELOID CELLS PROMOTES ANGIOGENESIS THROUGH VEGF AND S100A8

Ahn et al., PNAS (2014), 111(7), 2698-2703

May 4, 2010

INHIBITION OF MAC-1 (CD11B/CD18) ENHANCES TUMOR RESPONSE TO RADIATION BY REDUCING MYELOID CELL RECRUITMENT

  • Ahn et al., PNAS (2010), 107(18), 8363-8368

  • Introduced as research highlight at Nature Medicine

Mar 2008

MATRIX METALLOPROTEINASE-9 IS REQUIRED FOR TUMOR VASCULOGENESIS BUT NOT FOR ANGIOGENESIS: ROLE OF BONE MARROW-DERIVED MYELOMONOCYTIC CELLS

  • Ahn & Brown, Cancer Cell (2008), 13(3), 193-205

  • Introduced as research highlight

Jun 14, 2006

RADIOLYTIC AND CELLULAR REDUCTION OF A NOVEL HYPOXIA-ACTIVATED COBALT (III) PRODRUG OF A CHLOROMETHYLBENZINDOLINE DNA MINOR GROOVE ALKYLATOR

Ahn et al., Biochem Pharmacol (2006), 71(12), 1683-1694

Sep 2004

OPTIMIZATION OF THE AUXILIARY LIGAND SHELL OF COBALT(III)(8-HYDROXYQUINOLINE) COMPLEXES AS MODEL HYPOXIA-SELECTIVE RADIATION-ACTIVATED PRODRUGS

Ahn et al., Radiat Res (2004), 162(3), 315-325

LAB MEMBERS

Current lab members

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G-ONE AHN, PH.D.

PI (Lab Head)

  • 2019.09~present: Associate Professor, Seoul National University College of Veterinary Medicine

  • 2020.03~present: Adjunct Professor, Seoul National University College of Medicine (Cancer Biology Collaborative Course)

  • 2017.03~2017.09: Visiting Professor, Stem Cell Institute and Regenerative Medicine, Stanford University School of Medicine (Irving L. Weissman's lab)

  • 2015.09~2019.08: Associate Professor, Integrative Biosciences Biotechnology(IBB)/Life Science, POSTECH (Pohang University of Science and Technology)

  • 2011.04~2015.08: Assistant Professor, IBB, POSTECH

  • 2008.09~2011.03: Research Associate, Stanford University School of Medicine (Dept Radiation Oncology, J. Martin Brown's lab)

  • 2003.09~2008.08: Postdoc, Stanford University School of Medicine (Dept Radiation Oncology, J. Martin Brown's lab)

  • 2000.03~2003.08: Ph.D. in Pathology, The University of Auckland, Auckland, New Zealand (Auckland Cancer Society Research Centre, William R. Wilson's lab)

  • 1998.03~2000.02: M.Sc. (Hons) in Pharmacology, The University of Auckland, Auckland, New Zealand (Mark J. McKeage's lab)

  • 1995.03~1998.02: B.Sc. in Chemistry & Pharmacology, The University of Auckland, Auckland, New Zealand

ALUMNI

Past lab members
(students & postdocs only)

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Ye-Rim Lee M.S

  • 2022.02.28 ~ present : Graduate student (MS combined)

  • 2021.09.01~2022.02.28 : Undergraduate Student Researcher, College of Veterinary Medicine, Seoul National University

  • 2021.12.21~2020.02.05: Internship student, College of Medicine (Tumor Biology Collaborative Course), Seoul National University

  • 2017.03~2022.02 : Undergraduate student (Biology major), Department of Life Science, Yonsei University

RECRUITING INFORMATION

Graduate students, postdocs, and more!

Open for 2024 Fall Semester Admission

GRADUATE STUDENTS

We are actively recruiting graduate students for MS-PhD combined or PhD degrees. If interested, please e-mail us with your transcript and CV.

Currently Closed

POSTDOCTORAL FELLOWS

We are also actively recruiting postdoctoral research fellows. Those whom have biomedical expertise (Cancer Biology, Immunology, Cell Biology, Molecular Biology, Radiation Biology, Medicine, Veterinary Medicine) are particularly welcome (but not necessary). All fields of expertise are encouraged. If interested, please email your CV with a list of publications.

Currently Closed

RESEARCHERS

We hire full-time research technicians. Experience in Cell Biology, Molecular Biology, in vivo mouse handling are particularly preferred but not necessary.

Open

INTERNSHIP

We welcome undergraduate students for internship in the lab. They will experience various aspects of research - performing, writing, reading, and designing research by participating various activities in the lab.
If you're interested, you should write to PI to inquire the application process.

NEWS AND EVENTS

OUR 2019 CANCER RESEARCH PAPER RECEIVES THE MOST-CITED PAPER AWARD FROM AACR!

Posted: Feb 8th, 2021

Our 2019 Cancer Research paper, 'Tumor-associated macrophages enhance tumor hypoxia and aerobic glycolysis' was one of the most highly cited Cancer Research articles published in 2019! This will be highlighted as 'The Best of the AACR Journals' in upcoming AACR virtual annual meeting 2021 in May. Those of you who attend the AACR meeting this year, please check out our paper being highlighted!

My profile will also be highlighted in May at the following website:

the AACR Journals website

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LAB SOCIAL / DINING OUT!

Once a month get together to dine out

  • 12.18.19 - Lunch @ La Cucina, SNU

  • 01.16.20 - Dinner @ BBQ, NakSungDae

  • 03.17.20 - Lunch @ SoDamMaRu, SNU

  • 04.29.20 - Lunch @ Syaban, SNU

  • 07.03.20 - Dinner @ BBQ Cafe, SNU

  • 07.24.20 - Dinner @ Jeju Black pork BBQ, NakSungDae

  • 10.08.20 - Dinner @Wow steak house, NakSungDae

  • 10.12.20 - Lunch @ the Faculty Club, SNU

  • 12.24.20 - Lunch, take out from Italy SangHuae (Pasta & Pizza), Seminar room 412, Building 81

  • 01.07.21 - Take out lunch (Welcoming for internship students) from Italy SangHuae

  • 02.02.21 - Farewell dinner for internship students @ Wow steak house

  • 05.11.21 - Lunch @ Korean BBQ, NakSeongDae

  • 07.26.21 - Lunch @ the Faculty Club, Seoul National University

  • 08.13.21 - Lunch @ Korean BBQ, NakSeongDae

  • 08.31.21 - Lunch @ the Faculty Club, Seoul National University

More
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WE HAVE MOVED!

Posted Sep 2020

Aug 2nd, 2020
We've moved from building 85 to building 81!
Checkout the latest mailing address:
Lab: Rm 421, Building 81
Office: Rm 414, Building 81

More
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FACS (FLUORESCENCE-ACTIVATED CELL SORTER) MACHINE IN THE LAB!

Arrived Jan, 2021 from US!

Now we have a new FACS machine in the lab!!
No compensation is needed - Analyzing the whole spectra of a fluorochrome. With this technology, we can look up to 20 different fluorescence!
Doesn't Hyo Jae look happy with FACS? ^_^

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CANCER RESEARCH INSTITUTE, COLLEGE OF MEDICINE, SEOUL NATIONAL UNIVERSITY

Posted Sep 2021

Our lab is one of the participating research laboratories at Cancer Research Institute, College of Medicine, Seoul National University.
https://cri.snu.ac.kr/
Check out our lab at SNU CRI website by clicking 'more' button below.

LAB PHOTOS

Social photos

24.05.16 방사선 생명과학회 - 강기수 포스터상 수상

24.05.16 방사선 생명과학회 - 강기수 포스터상 수상

24.05.16-17 Korean Society of Radiation Bioscience - Gi-Sue Kang award

24.05.16-17 방사선 생명과학회 춘계학술대회

24.05.16-17 방사선 생명과학회 춘계학술대회

24.05.16-17 Korean Society of Radiation Bioscience Spring conference

24.03.15 Xstrahl 회전 방사선 기기 세미나

24.03.15 Xstrahl 회전 방사선 기기 세미나

24.03.15 Xstrahl - Rotating Radiation Equipment Seminar

24.02.26 예림 졸업-2

24.02.26 예림 졸업-2

24.02.26 Ye-Rim Lee graduation

24.02.26 예림 졸업

24.02.26 예림 졸업

24.02.26 Ye-Rim Lee graduation

23.12.03 남에스더 선생님 생일

23.12.03 남에스더 선생님 생일

23.12.03 Esther Nam birthday

23.11.10 방사선 생명과학회 - 이예림 우수구연상 수상 - 2

23.11.10 방사선 생명과학회 - 이예림 우수구연상 수상 - 2

23.11.10 Korean Society of Radiation Bioscience Fall conference - Ye-Rim Lee Award - 2

23.11.10 방사선 생명과학회 - 이예림 우수구연상 수상

23.11.10 방사선 생명과학회 - 이예림 우수구연상 수상

23.11.10 Korean Society of Radiation Bioscience Fall conference - Ye-Rim Lee Award

23.11.17 졸업생 Dr.복서연

23.11.17 졸업생 Dr.복서연

23.11.17 Interaction with graduate Dr.Seoyeon Bok

23.11.17 졸업생 Dr.복서연 교류

23.11.17 졸업생 Dr.복서연 교류

23.11.17 Interaction with graduate Dr.Seoyeon Bok

23.11.14 Prof.Amato Giaccia & Dr.Ejung Moon 교류

23.11.14 Prof.Amato Giaccia & Dr.Ejung Moon 교류

23.11.14 Interaction with Prof.Amato Giaccia & Dr.Ejung Moon

Nature metabolism 저널의 Chief editor. Christoph와 교류 - 한강불꽃축제

Nature metabolism 저널의 Chief editor. Christoph와 교류 - 한강불꽃축제

23.10.07 Fireworks in Han river with Christoph, Chief Editor Of Nat Metab.

Nature metabolism 저널의 Chief editor. Christoph와 교류 - 점심 식사

Nature metabolism 저널의 Chief editor. Christoph와 교류 - 점심 식사

23.10.07 Fireworks in Han river with Christoph, Chief Editor Of Nat Metab.

23.10.24 대학원생 예림 생일

23.10.24 대학원생 예림 생일

23.10.24 YR birthday

TME members 일상

TME members 일상

TME members (daily)

2022 제주 신약학회

2022 제주 신약학회 -1

2022 KSBMB DDC academy in Jeju - 1

2022 제주 신약학회

2022 제주 신약학회 - 2

2022 KSBMB DDC academy in Jeju - 2

2022 제주 신약학회

2022 제주 신약학회 - 3

2022 KSBMB DDC academy in Jeju - 3

2022 제주 신약학회

2022 제주 신약학회 - 4

2022 KSBMB DDC academy in Jeju - 4

2022 제주 신약학회

2022 제주 신약학회 - 5

2022 KSBMB DDC academy in Jeju - 5

2022 제주 신약학회

2022 제주 신약학회 - 6

2022 KSBMB DDC academy in Jeju - 6

2022 제주 신약학회

2022 제주 신약학회 - 7

2022 KSBMB DDC academy in Jeju - 7

2022 제주 신약학회

2022 제주 신약학회 - 8

2022 KSBMB DDC academy in Jeju - 8

21.12.02 제주 방사선학회

21.12.02 제주 방사선학회

21.12.02 JKSR (Korean Society of Radiology) in Jeju

21.12.02 제주 방사선학회

21.12.02 제주 방사선학회 - 2

21.12.02 JKSR (Korean Society of Radiology) in Jeju - 2

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21.07.24 랩 회식

21.07.24 Dinner

ENQUIRY

서울시 관악구 관악로 1, 서울대학교 수의과대학 81동 414호 (Lab: 421호)
Rm 414 (Lab: Rm 421), Building 81
College of Veterinary Medicine
Seoul National University
1 Gwanak-ro, Gwanak-gu
Seoul 08826, Korea

02-880-1179

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